PCR Detection of Porcine circovirus type 2 DNA in Whole Blood, Serum, Oropharyngeal Swab, Nasal Swab, and Feces from Experimentally Infected Pigs and Field Cases.
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- SHIBATA Isao
- Zen-noh Institute of Animal Health
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- OKUDA Yo
- Zen-noh Institute of Animal Health
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- YAZAWA Shigeto
- Zen-noh Institute of Animal Health
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- ONO Masaaki
- Zen-noh Institute of Animal Health
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- SASAKI Takashi
- Zen-noh Tokyo Branch
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- ITAGAKI Mitsuaki
- Gunma Chubu Livestock Hygiene Service Center
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- NAKAJIMA Nobuaki
- Gunma Chubu Livestock Hygiene Service Center
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- OKABE Yukinobu
- Gunma Prefectural Federation Aid Mutual Agricultural Association
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- HIDEJIMA Isao
- Gunma Prefectural Federation Aid Mutual Agricultural Association
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Porcine circovirus type 2 (PCV2) shedding patterns were investigated by polymerase chain reaction (PCR) for the detection of PCV2 DNA, and the diagnostic suitability of a sample for the PCR was examined by using different types of samples. In the experimental infection, sixteen pigs were inoculated intranasally with PCV2. The samples, including oropharyngeal and nasal swabs, feces, whole blood and serum became positive for PCV2 DNA by PCR immediately after the inoculation, and almost all samples remained positive during the observation period, post-inoculation-day 70. Field samples were collected from 313 pigs in five different age groups. The overall percentages of positive samples in the whole blood, nasal swabs, and feces detected by PCR were 30.4%, 19.2%, and 20.4%, respectively. The frequency of positive samples increased after the nursery stages and reached a peak in the 3 to 4-month-old pigs. These results indicate that PCV2 infection may occur after weaning, that PCV2 DNA may be present in whole blood for a long period after infection, and that whole blood and serum are the most suitable sample types for the PCR analysis of PCV2. <br>
収録刊行物
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- The Journal of Veterinary Medical Science
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The Journal of Veterinary Medical Science 65 (3), 405-408, 2003
公益社団法人 日本獣医学会
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詳細情報 詳細情報について
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- CRID
- 1390282681401899648
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- NII論文ID
- 110003947943
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- NII書誌ID
- AA10796138
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- COI
- 1:CAS:528:DC%2BD3sXjtFKktLs%3D
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- ISSN
- 13477439
- 09167250
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- NDL書誌ID
- 6514136
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- PubMed
- 12679576
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可