Protectivity of an Immunoaffinity-Purified 39kDa Capsular Protein of Avian Pasteurella multocida in Mice

• ALI Hussam Al-haj

  Department of Veterinary Microbiology, Faculty of Veterinary Medicine, Nippon Veterinary and Animal Science University

• SAWADA Takuo

  Department of Veterinary Microbiology, Faculty of Veterinary Medicine, Nippon Veterinary and Animal Science University

• NODA Ken

  National Veterinary Assay Laboratory

抄録

A 39 kDa protein of avian Pasteurella multocida strain P-1059 (serovar A:3) was purified from a crude capsular extract by immunoaffinity chromatography by using a ligand of purified mouse monoclonal antibody to the 39 kDa capsular protein of the strain. Protective activity of the purified 39 kDa protein antigen was determined by inoculation of ddY mice twice with 25 or 125 μg of the protein and challenge-exposure with 10 or 50 LD₅₀ of strains P-1059 or X-73 (serovar A:1). The results showed that the antigen gave high protection (60 to 100%). These results indicated that the 39 kDa protein of avian P. multocida is a cross-protective antigen over serovars A:1 and A:3.<br>

収録刊行物

• Ｔｈｅ　Ｊｏｕｒｎａｌ　ｏｆ　Ｖｅｔｅｒｉｎａｒｙ　Ｍｅｄｉｃａｌ　Ｓｃｉｅｎｃｅ

  Ｔｈｅ　Ｊｏｕｒｎａｌ　ｏｆ　Ｖｅｔｅｒｉｎａｒｙ　Ｍｅｄｉｃａｌ　Ｓｃｉｅｎｃｅ 66 (12), 1603-1604, 2004

  公益社団法人 日本獣医学会

キーワード

• active cross-protection
• avian <i>Pasteurella multocida</i>
• 39 kDa capsular protein

詳細情報

• CRID: 1390282681401412992
• NII論文ID: 110003963659
• NII書誌ID: AA10796138
• DOI: 10.1292/jvms.66.1603
• ISSN: 13477439
• ISSN: 09167250
• PubMed: 15644617
• Web Site: http://www.jstage.jst.go.jp/article/jvms/66/12/66_12_1603/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可