犬ジステンパーウイルスの犬悪性組織球症由来CCT細胞における増殖特徴

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  • YAMAGUCHI Ryoji
    Department of Veterinary Pathology, Faculty of Agriculture, University of Miyazaki
  • KOJIMOTO Atsuko
    Department of Veterinary Pathology, Faculty of Agriculture, University of Miyazaki
  • SAKAI Hiroki
    Department of Veterinary Pathology, Faculty of Agriculture, Gifu University
  • UCHIDA Kazuyuki
    Department of Veterinary Pathology, Faculty of Agriculture, University of Miyazaki
  • SUGANO Sumio
    The Institute of Medical Science, The University of Tokyo
  • TATEYAMA Susumu
    Department of Veterinary Pathology, Faculty of Agriculture, University of Miyazaki

書誌事項

タイトル別名
  • Growth Characteristics of Canine Distemper Virus in a New Cell Line CCT Cells Originated from Canine Malignant Histiocytosis

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Canine distemper virus (CDV) growth and the morphological characterization were examined in a cell line established from a canine malignant histiocytosis (CCT cell line). The susceptibility of the CCT cells to 3 CDV strains, FXNO, YSA-TC and MD-77 was shown by detection of the antigen in the indirect fluorescent assay. After passaging 4 and 9 times through the CCT cells, only FXNO strain could produce the syncytia where demonstrated the antigens. Titers of 9 passaged viruses through the CCT cells showed slightly higher in the CCT cells than those in Vero cells. Morphological characterization of karyorrhexis and specific DNA ladder by extracted DNA electrophoresis indicated apoptosis in the CDV infected CCT cells.<br>

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