Cloning and Expression of Goat Interleukin-18 Gene

DOI Web Site Web Site PubMed 参考文献14件
  • LIU Wen-qiang
    College of Veterinary Medicine, Shandong Agricultural University
  • ZHAO Hong-kun
    College of Veterinary Medicine, Shandong Agricultural University Milch Cow Research Center of Agricultural Science Academy of Shandong Province Jinan
  • GAO Yun-dong
    Milch Cow Research Center of Agricultural Science Academy of Shandong Province Jinan
  • ZHONG Ji-feng
    Milch Cow Research Center of Agricultural Science Academy of Shandong Province Jinan

この論文をさがす

抄録

We isolated and sequenced a 480 bp cDNA encoding mature goat interleukin-18 (gIL-18) from alveolar macrophages and splenocytes activated with LPS by RT-PCR. The gIL-18 gene was cloned into pET32a (+) vectors and sequenced. Nucleotide sequence of gIL-18 shares high homology with cattle. Fusional expression with pET32a (+) of gIL-18 of about 38kD was obtained by SDS-PAGE analysis after induction by IPTG in the E. Coli BL21 expression system. The recombinant protein can induce IFN-γ production in PBMC. The IL-18 mRNA was constitutively detected in goat alveolar macrophages with or without LPS, While, enhanced expression was detected in splenocytes and liver cells if treated by LPS, and can be weakly detected in Peripheral blood mononuclear cells (PBMCs) treated by activators. Significant deference of IL-18 mRNA level may reflect the capacity to produce mature IL-18 in such tissues.<br>

収録刊行物

参考文献 (14)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ