免疫磁気ビーズおよび ATP 法を用いた Lawsonia intracellularis の検出

DOI 日本農学文献記事索引 Web Site Web Site PubMed 被引用文献1件 参考文献24件
  • WATARAI Masahisa
    Department of Applied Veterinary Science, Obihiro University of Agriculture and Veterinary Medicine
  • YAMATO Yosuke
    Department of Applied Veterinary Science, Obihiro University of Agriculture and Veterinary Medicine
  • MURAKATA Kayo
    Department of Pathological Science, Obihiro University of Agriculture and Veterinary Medicine
  • KIM Suk
    Department of Applied Veterinary Science, Obihiro University of Agriculture and Veterinary Medicine
  • OMATA Yoshitaka
    Department of Basic Veterinary Science, Obihiro University of Agriculture and Veterinary Medicine
  • FURUOKA Hidefumi
    Department of Pathological Science, Obihiro University of Agriculture and Veterinary Medicine

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タイトル別名
  • Detection of Lawsonia intracellularis Using Immunomagnetic Beads and ATP Bioluminescence

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Lawsonia intracellularis is an obligate intracellular pathogenic bacterium that causes proliferative enteropathy in various animals. The detection of L. intracellularis in clinical and environmental samples is necessary for the diagnosis of infection and epidemiological investigations. For the detection of L. intracellularis in fecal samples, we have developed an immunological method using immunomagnetic separation and ATP bioluminescence. Magnetic beads were coated with an anti-Lawsonia surface antigen (LsaA) antibody in order to capture the L. intracellularis in fecal samples from infected rabbits and the bacteria captured by the immunomagnetic beads were assayed by means of ATP bioluminescence. Our results showed that L. intracelluraris was detected by immunomagnetic separation of bacteria-holding magnetic beads and ATP-based bioluminescence, suggesting that our methods could be useful for the diagnosis of proliferative enteropathy.<br>

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