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- MAEDA Ken
- Department of Veterinary Microbiology, Faculty of Agriculture, Yamaguchi University
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- KAI Kazushige
- Department of Veterinary Microbiology, Faculty of Agriculture, Yamaguchi University
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- MATSUMURA Tomio
- Epizootic Research Station, Equine Research Institute, Japan Racing Association
書誌事項
- タイトル別名
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- Genomic Diversity among Equine Herpesvirus-4 Field Isolates
この論文をさがす
抄録
Infection with equine herpesvirus-4 (EHV-4) is a major cause of respiratory tract disease, equine rhinopneumonitis, in horses. Although the full sequence of EHV-4 has been reported, genomic differences among EHV-4 field isolates have not yet been characterized. In this study, the genomic diversity between 23 Japanese EHV-4 isolates was analyzed by digestion with restriction endonucleases (BamHI, BgIII, EcoRI, SacI, and SalI) and polymerase chain reaction (PCR). The restriction endonuclease digestion patterns of the EHV-4 field isolates showed distinct differences which included mobility shifts of some fragments as well as loss and/or gain of fragments. Two EHV-4 genes containing repeat sequences, ORFs 24 and 71, were amplified by PCR and the amplified fragments were compared among the field isolates. The sizes of the amplified fragments varied among epizootiologically unrelated isolates, while the fragments of related isolates had the same size. The observed genomic diversity among EHV-4 field isolates may be a useful tool for epidemiological study of equine rhinopneumonitis by EHV-4 infection.<br>
収録刊行物
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- The Journal of Veterinary Medical Science
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The Journal of Veterinary Medical Science 67 (6), 555-561, 2005
公益社団法人 日本獣医学会
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詳細情報 詳細情報について
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- CRID
- 1390282681401572992
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- NII論文ID
- 130000448257
- 110003963769
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- NII書誌ID
- AA10796138
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- ISSN
- 13477439
- 09167250
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- NDL書誌ID
- 7403864
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- PubMed
- 15997181
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- IRDB
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可