再植歯の整復・固定後の修復過程および歯の移動に対するb-FGFの影響に関する病理組織学的研究

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  • 下田 哲也
    福岡歯科大学大学院歯学研究科歯科矯正学専攻

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  • Histopathological Study on Effects of b-FGF on Healing rocess of Periodontal Tissue after Fixation of Replanted Teeth and their Movement

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In orthodontics, malpositioned replantation teeth are required to move to a correct position. However, after replantation, such teeth often cause bony fusion, resulting in non-movement and root resorption. Recombinant basic fibroblast growth factor (b-FGF) was applied to observe the prognosis and its effect on replanted tooth movement. Maxillary lateral incisors of 30 beagle dogs were used and kept in a saline-soakedga uze for 60 min and replanted after application of b-FGF drips (FGF group) or without this treatment (control group). The Control and FGF groups were divided into 6 subgroups. Replanted teeth of each group were fixed was carried out for 3,5,7,21 or 35 days after tooth replantation and tooth transfer of a group after fixation. In the FGF group, b-FGF (50 μg) was applied to the entire root surface using a Hamilton syringe before replantation. Fixation was performed for 1 week, 4 weeks after tooth replantation. The replanted tooth was lingually transferred for 28 days. The replanted tooth was observed by the X-ray examination. The length of lingual transfer was measured 28 days often applcation of the apparatus. After the experiment, the teeth were histopathologically observed by HE staining and immunohisto-chemical staining using the PCNA antibody. The Results were as follows : 1.Dental X-ray findings : A translucent image of thepe riodontal membrane and the lamina dura were observed at 3〜7 days fixation. 21 days after fixation, the periodontal cavity had partly disappeared in the control group. After 35 days, extensive disappearance of the periodontal cavity and surfacere sorption of the root were observed in the control group. After tooth transfer, the control group showed disappearance of the periodontal cavity and surface resorption of the root, and the FGF group showed dilatation of the periodontal cavity. 2.Length of transfer : The mean length of transfer was 0.17 mm in the control group and 0.67 in the FGF group with a significant differrence. 3.Findings of HE staining : 1) Fixation group : 3 days after fixation, both control and FGF groups showed irregular interruption of the remaining periodontal membrane and many clots. After 5 days, the periodontal membrane was interrupted, but a number of new periodontal membrane fibers and clear arrangement were observed in the FGF group when compared to the control group. After 7 days, major fiber bundlesin the alveolar cavity were markedly repaired, and interruption was unclear in the FGF group when compared to the control group. After 21 days, the control group showed new periodontal membrane fibers in the entire area and partial osseous adhesion. In the FGF group, the arrangement of new periodontal membrane fibers was close to normal. After 35 days, the buccal root surface of both groups was close to normal images. In the control group, extensive osseous adhesion and rootres orption were observed on the lateral surface of the palate. In the FGF group, osseous adhesion and root resorption were partially observed, but their extent wassm aller than that in the control group. 2) Transfer groups The control group showed extensive osseous adhesion and partial root resorption on both compression and tention sides. The FGF group showed bone resorption by osteoclasts in the alveolar bone surface and partial root resorption on the compression side. On the tention side, the periodontal membrane was regularly arranged, and osteoblasts onthe alveolar bone surface and addition of new bone associated with transfer were observed. 4.Findings of immunostaining : 3 dyas after fixation, the mean positive cell count was 17 in the control group and 38.3 in the FGF group with a significant differrence. But it wasn't ovserved after 5 days with a significant differrence. It is suggested that drip application of b-FGF after replantation of dislocated teethre sulted in early regeneration of periodontal membrane fibers, less abnormalities and good repair of the periodontal tissue. After ort

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