Maintenance of a Round Cell Shape and Physiological Responsiveness to Insulin of Mature Adipocytes by Culturing in Type I Collagen Gel :

• HARIMOTO,Masami

  Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo

• HIROSE,Motohiro

  Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo

• HAYASHI,Toshihiko

  Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo

• ATOMI,Yoriko

  Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo

抄録

Cellular behavior including viability, cell shape, and insulin responsiveness was comparedamong three culture systems of mature adipocytes obtained from rat adipose tissues : in type I collagen gel, on type I collagen-coated dish, and floating in culture medium. Rat mature adipocytes cultured in the type I collagen gel maintained a round shape with terminal differentiation state as long as 50 days or more. The adipocytes retained a responsiveness to insulin as shown by leptin secretion. The multicellular organization comprising type I collagen gel and mature adipocytes appears functionally and structurally to be an adipose tissue-like construct.

収録刊行物

• Connective tissue

  Connective tissue 32 (1), 11-15, 2000-03-25

  日本結合組織学会

キーワード

• mature adipocyte
• culture system
• type I collagen gel
• cell shape
• physiological function

詳細情報

• CRID: 1541417145300138752
• NII論文ID: 110004002281
• NII書誌ID: AN10169901
• Web Site: http://dl.ndl.go.jp/info:ndljp/pid/11084778
• Web Site: https://search.jamas.or.jp/link/ui/2000220885
• 本文言語コード: en
• データソース種別:
  • NDL-Digital
  • CiNii Articles