Ｐｏｒｐｈｙｒｏｍｏｎａｓ　ｇｉｎｇｉｖａｌｉｓの歯冠修復材への付着に及ぼす唾液中フィブロネクチンの作用

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  • Functional Role of Fibronectin in Saliva on Adherence of Porphyromonas gingivalis to Restorative Materials.

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The adherence of oral bacteria to host cells and to restorative materials is considered to be an important first step in the development of periodontal tissue destruction. Bacterial fimbriae are one of the major cell structures for this adherence. Since bacterial adherence to these materials is mediated by specific molecules in the saliva, it is very important to investigate substances that modulate fimbria-mediated binding to saliva-coated materials and to determine the mechanism of action. Porphyromonas gingivalis is one of the predominant pathogen in adult periodontitis. Fimbriae are an important cell structure that involved in the adherence of the organism to host cells. In this study, we examined the involvement of fibronectin in saliva for P. gingivalis fimbria-mediated adherence to the restorative materials.<BR>Three different highly filler-filled resin-composite materials, self-cured acrylic resin (RTPR) and a cast 20 K gold alloy were tested. P. gingivalis adherence to the restorative materials was measured by an enzyme-linked immunosorbent assay (ELISA) with its monoclonal antibody. The adherence to the restorative materials was dramatically inhibited by monoclonal antibody against its fimbriae. The fimbriamediated adherence increased when the materials were pretreated with human parotid saliva. This increase in the bacterial adherence to saliva-pretreated materials was inhibited by pretreatment with fibronectin antibody. Purified human fibronectin pretreatment significantly increased the fimbria-mediated adherence to the restorative materials. The present study demonstrates that P. gingivalis fimbria-mediated adherence to the restorative materials is regulated by fibronectin in saliva. J. Jpn. Soc. Periodontol., 42: 235-246, 2000.

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