歯周組織創傷治癒過程におけるエナメルマトリックスタンパク質の上皮細胞の動態に及ぼす影響

DOI 被引用文献2件 参考文献43件
  • 川井 英敬
    東京歯科大学大学院歯学研究科歯科保存学第二講座

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タイトル別名
  • Epithelial Cell Kinetics of Periodontal Wound Healing after Application of Enamel Matrix Proteins.

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The present study was designed to examine the effects of enamel matrix proteins (EMP) on the proliferation of the junctional epithelial cells during wound healing in periodontal tissues. Proliferative cell nuclear antigen (PCNA) was immunohistochemically used to detect proliferative activity of epithelial cells, and immunolocalization of laminin was also demonstrated to evaluate the distribution of basement membrane during periodontal tissue healing. Forty Wistar Rats were used in this study. The palatal bone of the first molar was removed, and the exposed root surface was carefully planed to remove the cementum. The exposed root was conditioned for 15 s by means of cotton pellets soaked in a saturated solution of citric acid (pH 1) and then carefully rinsed with sterile physiologic saline. EMP was applied to the treated root surface and left first molar (experimental group), but no EMP was applied to the right first molar (control group). The flap was repositioned. On 1, 2, 3, 4 and 8 weeks after the treatment, the animals were sacrificed and block sections taken from the jaws. Tissue sections were stained with hematoxylin-eosin (H-E), and immunohistochemical. reactivities for PCNA and laminin were examined. Epithelial downgrowth was decreased in the experimental group, which was statistically significant (p<0.05) from posttreated week 1 to 8. PCNA positive cells in epithelial cells was higher in the control group than those in the experimental group from postoperative week 1 to 4, with a highly significant difference observed in weeks 3 and 4. From week 1 to 4, immuno-reactivity for laminin in the epithelium was not observed in the experimental group, while the activity was obvious in earlier stages in the control group. The results of this study suggest that EMP may act as epithelial cytostatic agent, and inhibit the growth of epithelial cells. J. Jpn. Soc. Periodontol., 43: 396-408, 2001.

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