Cadmium Sensitivity and Defense Mechanism to Cadmium Exposure in Human Gingival and Periodontal Ligament Fibroblasts in vitro

DOI 1 Citations 45 References
  • KAMOI Hatsuko
    Department of Preventive and Community Dentistry, The Nippon Dental University, School of Dentistry at Tokyo

Bibliographic Information

Other Title
  • ヒト歯肉および歯根膜由来線維芽細胞のカドミウム感受性とカドミウム毒性に対する防御機構について

Search this article

Abstract

The present research investigated mechanism of protection against cadmium (Cd) sensitivity and Cd toxicity in human oral tissue using cells isolated from human gingiva and periodontal membrane. The results obtained are as follows. 1. Cell growth and DNA synthesis were inhibited in fibroblasts isolated from adult gingiva and periodontal membrane as well as infant gingiva at Cd concentrations of 10-5 mM and above. There were no obvious differences in Cd sensitivity according to cell type. 2. The intracellular concentration of Cd in cells exposed to Cd for 24 hours was lower (10-7〜10-5 mM Cd) than that in control group for all cell types and was highest in the 10-3 and 10-1 mM Cd concentration groups. 3. The intracellular concentration of GSH in cells exposed to Cd for 24 hours was high in the group with a high intracellular concentration of Cd. 4. Induced synthesis of MT by Cd was observed for 12 hours of exposure in all groups up to a Cd concentration of 10-3 mM, and was even greater at 24 hours. In contrast, MT induction was not observed in any cell type at a concentration of 10-1 mM. 5. Tissue staining with fluorescent antibody revealed that MT was located mainly in the nucleus in all three types of cells. 6. MT-II mRNA expression was observed in human periodontal membrane-derived fibroblast cells exposed to Cd for 6 hours. Based on the above findings, it is concluded that there were no clear differences in the sensitivities to Cd of adult gingival cells, adult periodontal membrane-derived fibroblasts and infant gingival fibroblasts. The results also suggest that the production of both MT and GSH are promoted by Cd exposures in all types of cells studied. Finally, MT-II mRN A was detected in adult periodontal membrane-derived fibroblasts exposed to Cd, indicating that MT- II, as an isotype of MT induced in these cells, is present.

Journal

Citations (1)*help

See more

References(45)*help

See more

Keywords

Details 詳細情報について

Report a problem

Back to top