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The dipteran family Rhinophoridae is recorded for the first time from Japan. Acompomintho lobata VILLENEUVE, 1927 is recorded from Ryukyu Islands, and Oplisa japonica sp. n. is described from Kyushu and southern Honshu. A key is provided to identify both species of Rhinophoridae and to separate these from other Japanese members of the Tachinidae family-group (Oestroidea).