The Combination of Suicide Gene Therapy and Radiation Enhances the Killing of Nasopharyngeal Carcinoma Xenographs
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- XIA Jiahui
- National Laboratory of Medical Genetics of China, Central South University
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- XIA Kun
- National Laboratory of Medical Genetics of China, Central South University
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- FENG Yong
- E.N.T Department of Xiangya Hospital, Central South University
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- TANG Aifa
- National Laboratory of Medical Genetics of China, Central South University
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- TANG Yaoyun
- E.N.T Department of Xiangya Hospital, Central South University
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- WU Lingqian
- National Laboratory of Medical Genetics of China, Central South University
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- LIANG Desheng
- National Laboratory of Medical Genetics of China, Central South University
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- CAI Fang
- National Laboratory of Medical Genetics of China, Central South University
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- PAN Qian
- National Laboratory of Medical Genetics of China, Central South University
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- LONG Zhigao
- National Laboratory of Medical Genetics of China, Central South University
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- DAI Heping
- National Laboratory of Medical Genetics of China, Central South University
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- ZHANG Yunyi
- E.N.T Department of Xiangya Hospital, Central South University
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- ZHAO Suping
- E.N.T Department of Xiangya Hospital, Central South University
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- CHEN Zhuchu
- Xiangya Hospital of Central South University
Bibliographic Information
- Other Title
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- Combination of Suicide Gene Therapy and Radiation Enhances the Killing of Nasopharyngeal Carcinoma Xenographs
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Abstract
Nasopharyngeal carcinoma (NPC) is very common in Southern China and Southeast Asian countries. To explore a novel and more effective approach to NPC therapy, a combined strategy of suicide genes and radiation was designed in this study. Five suicide gene expression cassettes, yeast CD, yeast CD/UPRT, and yeast CDglyTK gene controlled by CMV, and Egr-1 and a synthetic CMV-enhanced Egr-1 promoter (CE) were constructed in an expression vector p11MS. The expression of suicide genes in NPC CNE-2 cells were detected by RT-PCR and Western blot. The cytotoxicity of suicide gene therapy and radiation were analyzed by MTT assay. An animal study in which yeast CD/UPRT-expressing CNE-2 tumors in nude mice were treated with 5-FC and radiation was also developed. Our results revealed that p11MSCEyCD/UPRT and p11MSCEyCDglyTK are superior over three other constructs in the killing of NPC cells in vitro. We combined suicide gene-expressing tumors, 5-FC treatment, and radiation in vivo and found that the tumors greatly regressed, some disappeared completely in 3 nude mice in the yCD/UPRT group, and a significant difference of tumor volumes was observed between this group and the other four groups (p < 0.05). Our results indicated that suicide gene therapy and radiation have a synergic effect on NPC therapy, and the combined strategy of radiogene therapy is of great potential as a substitute for the traditional method, radiation alone, in NPC therapies.<br>
Journal
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- Journal of Radiation Research
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Journal of Radiation Research 45 (2), 281-289, 2004
Journal of Radiation Research Editorial Committee
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Details 詳細情報について
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- CRID
- 1390282680193067648
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- NII Article ID
- 110004041028
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- NII Book ID
- AA00705792
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- ISSN
- 13499157
- 04493060
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- NDL BIB ID
- 7014565
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- PubMed
- 15304972
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- NDL-Digital
- CiNii Articles
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- Abstract License Flag
- Disallowed