Potential in a Single Cancer Cell to Produce Heterogeneous Morphology, Radiosensitivity and Gene Expression

• BAN Sadayuki

  RadGenomics Project, Frontier Research Center, National Institute of Radiological Sciences

• ISHIKAWA Ken-ichi

  RadGenomics Project, Frontier Research Center, National Institute of Radiological Sciences

• KAWAI Seiko

  RadGenomics Project, Frontier Research Center, National Institute of Radiological Sciences

• KOYAMA-SAEGUSA Kumiko

  RadGenomics Project, Frontier Research Center, National Institute of Radiological Sciences

• ISHIKAWA Atsuko

  RadGenomics Project, Frontier Research Center, National Institute of Radiological Sciences

• SHIMADA Yutaka

  Department of Surgery & Surgical Basic Science, Graduate School of Medicine, Kyoto University

• INAZAWA Johji

  Institute for Molecular Cytogenetics, Tokyo Medical and Dental University

• IMAI Takashi

  RadGenomics Project, Frontier Research Center, National Institute of Radiological Sciences

抄録

Morphologically heterogeneous colonies were formed from a cultured cell line (KYSE70) established from one human esophageal carcinoma tissue. Two subclones were separated from a single clone (clone13) of KYSE70 cells. One subclone (clone13-3G) formed mainly mounding colonies and the other (clone 13-6G) formed flat, diffusive colonies. X-irradiation stimulated the cells to dedifferentiate from the mounding state to the flat, diffusive state. Clone 13-6G cells were more radiosensitive than the other 3 cell lines. Clustering analysis for gene expression level by oligonucleotide microarray demonstrated that in the radiosensitive clone13-6G cells, expression of genes involved in cell adhesion was upregulated, but genes involved in the response to DNA damage stimulus were downregulated. The data demonstrated that a single cancer cell had the potential to produce progeny heterogeneous in terms of morphology, radiation sensitivity and gene expression, and irradiation enhanced the dedifferentiation of cancer cells.<br>

収録刊行物

• Journal of Radiation Research

  Journal of Radiation Research 46 (1), 43-50, 2005

  Journal of Radiation Research 編集委員会

キーワード

• Dedifferentiation
• Morphology
• Radiosensitivity
• Gene expression
• Microarray

詳細情報

• CRID: 1390282680193518080
• NII論文ID: 110004041094
• NII書誌ID: AA00705792
• DOI: 10.1269/jrr.46.43
• ISSN: 13499157
• ISSN: 04493060
• NDL書誌ID: 7290572
• PubMed: 15802858
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I7290572
• Web Site: http://academic.oup.com/jrr/article-pdf/46/1/43/2738735/jrr-46-43.pdf
• Web Site: http://dl.ndl.go.jp/info:ndljp/pid/10918643
• Web Site: https://search.jamas.or.jp/link/ui/2006178809
• 本文言語コード: en
• データソース種別:
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  • NDL
  • Crossref
  • PubMed
  • NDL-Digital
  • CiNii Articles
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