社団法人日本分析化学会 分析化学 05251931 32 12 E379 E386 1983-12-05 1983-12-05 COLOR REACTION BETWEEN PYROGALLOL RED-MOLYBDENUM (VI) COMPLEX AND PROTEIN FUJITA,Yoshikazu MORI,Itsuo KITANO,Shoko The Japan Society for Analytical Chemistry Japan analyst A color reaction between pyrogallol red(PR) - molybdenum(VI) complex and protein was investigated. A spectrophotometric method using the PR-molybdenum(VI) complex could be used in the concentration range of 0-400 μg/lO ml of albumin, where the Sandell sensitivity was calculated to be 0.0048 μg/cm^2 for albumin at 600 nm. A detection test of protein on a spot plate was also studied. The proposed method was applied to the determination of urinary protein; analytical results were satisfactory. FUJITA,Yoshikazu Osaka College of Pharmacy MORI,Itsuo Osaka College of Pharmacy KITANO,Shoko Osaka College of Pharmacy