β-1.4-マンノビオースを用いたβ-マンノシダーゼ活性測定法

• 渡邉, 悟

• 牛澤, 良美

• 安井, 恒男

• ワタナベ, サトル

• ウシザワ, ヨシミ

• ヤスイ, ツネオ

• WATANABE, SATORU

• USHIZAWA, YOSHIMI

• YASUI, TSUNEO

書誌事項

タイトル別名

• Measurement of β-Mannosidase Activity Using β-1,4-Mannobiose
• ベータ 1 4 マンノビオース オ モチイタ ベータ マンノシダーゼ カッセイ

抄録

P(論文)

"Glucose C II - Test Wako" (Glucose Oxidase (GOD) Reagent) reacts glucose well, and is sensitive against xylose and mannose (M_1). Using this reagent and β-1, 4-mannobiose (M_2), Aspergillus niger β-mannosidase activily could be measured. The enzyme hydrolyzed M_2 to produce M_1, and no transfer product was detected in the thin-layer chromatography. Optimal pH of the enzyme using M_2 was measured to be the same around pH 3-4 as using p-nitrophenyl β-D-mannopyranoside (PNPM). The enzyme activity measured by the developed method seemed to be identical with the activity using PNPM as substrate. This result indicate that β-mannosidase activity can be measured by the developed method using M_2 instead of PNPM.

収録刊行物

• 聖徳栄養短期大学紀要

  聖徳栄養短期大学紀要 23 8-12, 1992-12-20

  東京 : 聖徳栄養短期大学

キーワード

• β-Mannosidase
• β-1,4-Manoobiose
• Mannose

詳細情報

• CRID: 1050845763400992128
• NII論文ID: 110004090340
• NII書誌ID: AN00128690
• ISSN: 02866366
• NDL書誌ID: 3827950
• Web Site: https://tsc.repo.nii.ac.jp/records/2072
• Web Site: http://id.ndl.go.jp/bib/3827950
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I3827950
• 本文言語コード: en
• 資料種別: departmental bulletin paper
• データソース種別:
  • IRDB
  • NDL
  • CiNii Articles