Localization of RANKL and cathepsin K, B and L in rat periodontal tissues during experimental tooth movement :

• AIHARA,Norihito

  Department of Orthodontics, Nihon University School of Dentistry at Matsudo

• OTSUKA,Aki

  Department of Orthodontics, Nihon University School of Dentistry at Matsudo

• YAMAGUCHI,Masaru

  Department of Orthodontics, Nihon University School of Dentistry at Matsudo

• OKADA,Hiroyuki

  Department of Oral Pathology, Nihon University School of Dentistry at Matsudo

• UTSUNOMIYA,Tadahiko

  Department of Oral Pathology, Nihon University School of Dentistry at Matsudo

• YAMAMOTO,Hirotsugu

  Department of Oral Pathology, Nihon University School of Dentistry at Matsudo

• KASAI,Kazutaka

  Department of Orthodontics, Nihon University School of Dentistry at Matsudo

抄録

The differentiation and functions of osteoclasts are regulated by osteoblast-derived factors such as receptor activator of NFκB ligand (RANKL) that stimulates osteoclasts formation. The role of cathepsin K (CAK), cloned as a novel collagenolytic cysteine proteinase, cathepsin L (CAL) and cathepsin B (CAB) in bone resorption was investigated. A recent immunocytochemical study reported that CAB and CAL were localized in the periodontal ligament (PDL) of rat molars. The present study focused on the localization of RANKL, CAB, CAL and CAK during experimental tooth movement in rats, and then elucidates mechanism of tooth movement. Experimental tooth movement was accomplished for 1, 3 and 7 days using the Waldo method. To determine localization of RANKL, CAB, CAL and CAK proteins using immunohistochemistry. Immunoreactivity for RANKL became strong in osteoblasts, osteoclasts and PDL fibroblasts from 1 day after tooth movement. Further, CAB and CAL were detected in osteoclasts and PDL fibroblasts and CAK was detected in osteoclasts specifically. On 3 days after tooth movement, immunoreactivity of those cathepsins was increased in osteoclasts and fibroblasts. The present study results showed that immunoreactivity for RANKL became stronger with tooth movement. Further, cathepsins expression was increased in osteoclasts and fibroblasts later than RANKL expression. Therefore, RANKL, CAB, CAL and CAK may be involved in the process of bone resorption during tooth movement.

収録刊行物

• Orthodontic waves : journal of the Japanese Orthodontic Society : 日本矯正歯科学会雑誌

  Orthodontic waves : journal of the Japanese Orthodontic Society : 日本矯正歯科学会雑誌 64 (4), 107-113,

  日本矯正歯科学会

キーワード

• periodontal ligament
• osteoclast
• RANKL
• cathepsins

詳細情報

• CRID: 1543668945070495232
• NII論文ID: 110004436683
• NII書誌ID: AA11222509
• Web Site: http://dl.ndl.go.jp/info:ndljp/pid/10631732
• Web Site: https://search.jamas.or.jp/link/ui/2006295508
• 本文言語コード: en
• データソース種別:
  • NDL-Digital
  • CiNii Articles