ヘルペスウイルスプロテアーゼの活性制御機構と新たな阻害部位

• 榛葉 信久

  味の素（株）ライフサイエンス研究所

書誌事項

タイトル別名

• Regulating the Activity of the Herpesviral Protease and Its Viable Target for Inhibition
• ヘルペスウイルスプロテアーゼ ノ カッセイ セイギョ キコウ ト アラタ ナ ソガイ ブイ

抄録

Kaposi’s sarcoma-associated herpesvirus (KSHV), like all herpesviruses, encodes a protease (KSHV Pr), which is necessary for the viral lytic cycle. Herpesviral proteases function as obligate dimers, however, each monomer has an active site, which is spatially separate from the dimer interface. To address the potential of targeting the dimer interface, a 30 amino acid helical peptide was synthesized by protein grafting of an interfacial KSHV Pr a helix with a small stable protein, avian pancreatic polypeptide (aPP) to disrupt the dimerization of KSHV Pr. Biochemical analysis revealed that the rationally designed helical peptide inhibited KSHV Pr dimerization and activity. These results indicate that the dimer interface, as well as the active sites, of herpesvirus proteases is a viable target for inhibiting enzyme activity.<br>

収録刊行物

• 生物物理

  生物物理 46 (2), 87-92, 2006

  一般社団法人 日本生物物理学会

キーワード

• herpesvirus protease
• Kaposi’s sarcoma-associated herpesvirus
• dimer
• protein grafting

詳細情報

• CRID: 1390001206534524928
• NII論文ID: 110004455817
• NII書誌ID: AN00129693
• DOI: 10.2142/biophys.46.87
• COI: 1:CAS:528:DC%2BD28XjsFWns7c%3D
• ISSN: 13474219
• ISSN: 05824052
• NDL書誌ID: 7904331
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I7904331
• Web Site: http://www.jstage.jst.go.jp/article/biophys/46/2/46_2_87/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2006235718
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可