K-ras Mutations in Gallbladder Carcinomas in Patients with Anomalous Arrangement of the Pancreaticobiliary Duct

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  • ITOI T
    First Department of Pathology, Niigata University School of Medicine
  • Hidenobu Watanabe
    First Department of Pathology, Niigata University School of Medicine
  • Tatsuya Yoshikawa
    Department of Gastroenterology Surgery, Tokyo Women's Medical College

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Abstract

Mutation of the K-ras codon 12 was examined in 17 gallbladder carcinomas and 14 nonneoplastic gallbladder mucosa in patients with anomalous arrangement of the pancreaticobiliary duct (APBD), using nested polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis. In addition, we investigated the relationship between the K-ras mutation and cellular proliferation using Ki-67 (MIB1) monoclonal antibody. Mutation of K-ras codon 12 was found in 7 of 17 (41.2%) gallbladder carcinomas but not in nonneoplastic gallbladder mucosa. There was neither a correlation between the presence of the K-ras mutation and the size or depth of invasion of the carcinoma, nor any statistically significant difference in cellular proliferation of the same grade of cytologic atypia between tumors with and without K-ras mutation (Ki-67 labeling index, high grade; 35.2 ±13.3% and 31.6±13.9%, low grade; 14.2±4.9% and 21.2± 12.8%, respectively). This indicates that mutation of K- ras codon 12 may be involved in the development of gallbladder carcinomas preferentially in patients with APBD, but not in the progression of the Ki-67 labeling index, tumor size, or tumor invasion.

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