INTERLEUKIN-1 α INCREASES THE EXPRESSION OF INTEGRIN α_Vβ_3 IN HUMAN PANCREATIC CANCER CELLS

  • 栗本 昌明
    Department of Gastroenterological Surgery, Nagoya City University Graduate School of Medical Sciences
  • 沢井 博純
    Department of Gastroenterological Surgery, Nagoya City University Graduate School of Medical Sciences
  • 岡田 祐二
    Department of Gastroenterological Surgery, Nagoya City University Graduate School of Medical Sciences
  • 松尾 洋一
    Department of Gastroenterological Surgery, Nagoya City University Graduate School of Medical Sciences
  • 山本 稔
    Department of Gastroenterological Surgery, Nagoya City University Graduate School of Medical Sciences
  • 舟橋 整
    Department of Gastroenterological Surgery, Nagoya City University Graduate School of Medical Sciences
  • 竹山 廣光
    Department of Gastroenterological Surgery, Nagoya City University Graduate School of Medical Sciences
  • 真辺 忠夫
    Department of Gastroenterological Surgery, Nagoya City University Graduate School of Medical Sciences

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Background and Aims: Pancreatic cancer is refractory to surgical treatment because of its high metastatic potential. We investigated the effect of IL-1 α on the expression of integrin α_Vβ_3 and tumor-associated angiogenesis in pancreatic cancer cell lines. Methods: We used three pancreatic cancer cell lines. The expression of integrin α_Vβ_3, mRNA and protein levels was examined by RT-PCR and flow cytometric analysis. The alteration of integrin α_Vβ_3 expression was measured using CELISA after treatment with rIL-1 α. A coculture system of HUVEC and fibroblasts was used to investigate tumor-associated angiogenesis. Results: Integrin cuand β_3 subunits were detected in all three cell lines both at mRNA and protein levels. The effect of the enhancement on integrin α_Vβ_3 expression induced by rIL-1 α was detected only in BxPC-3 cells, which possess a high potential to metastasize to the liver. Furthermore, a function blocking antibody to the α_Vβ_3 integrin inhibited tube formation associated with BxPC-3 cells. Conclusions: IL-1 α can increase the expression of the integrin α_Vβ_3 only in the pancreatic cancer cell line that exhibits a high potential to metastasize to the liver. Furthermore, the blockade of integrin α_Vβ_3 can inhibit tumor-associated angiogenesis, in vitro. These results suggested that IL-1 a is an important mediator of pancreatic cancer progression and that treatment with antibodies against integrin α_Vβ_3 may improve the prognosis of pancreatic cancer.

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