Immunological responses of human peripheral blood mononuclear cells (PBMCs) derived from healthy donors cultured with β-1.3-1.6 glucan produced by Aureobasidium pullulans

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  • Immunological respnses of human peripheral blood mononuclear cells (PBMCs) derived from healthy donors cultured with β-1.3-1.6 glucan produced by Aureobasidium pullulans
  • Immunological responses of human peripheral blood mononuclear cells PBMCs derived from healthy donors cultured with ベータ 1 3 1 6 glucan produced by Aureobasidium pullulans

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Aureobasidium pullulansによって産生されるβ-1.3-1.6グルカンは、ヒト末梢血単核球のトリチウムサイミジンの取り込み(細胞増殖)を優位に増強させた。この細胞増殖の増強は、CD11a(lymphocyte-function associated antigen-1: LFA-1)に対するモノクローナル抗体(mNI-58A)で完全に抑制された。さらに、β-1.3-1.6グルカンはヒト末梢血単核球ののインターロイキン-8(IL-8)産生能も有意に増加された。以上の結果は、β-1.3-1.6グルカンがLFA-1(CD11a)分子の経路を介してヒト末梢血単核球に免疫能を付与したことを示すものである。

We have succeed in purifying a new β-1.3-1.6 gluchan produced by the black yeast Aureobasidium pullulans (A, pullulnas) FO68, and reported that this β-1.3-1.6 glucan has unique immunological actions such as interleukin-8 (IK-8) production in the human monocyte-like cell line U937 and the acquistion of natural killer (NK) activity in mouse peritoneal cells. In this study, we investigated some immunological responses of the β-1.3-1.6 glucan on human peripheral blood mononuclear cells (PBMCs) derived from healthy donors. The ^3H-thymidine incorporation rates (cell proliferation) of the human PBMCs cultured with β-1.3-1.6 glucan were significnatly enhanced, and this enhancement was completely blocked in the presene of the CD11a (lymphocyte-function associated antigen-1:LFA-1) monoclonal antibody, mNI-58A. Furthermore, production of IL-8 into the culture supernatants from the human PBMCs cultured with β-1.3-1.6 glucan was also significantly augmented. There fingings strongly indicate that the β-1.3-1.6 glucan produced by A, pullulans FO68 sufficiently enhanced some immune responses of human PBMCs.

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