ラットの角膜と網膜における steroid sulfatase の分布

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  • ラット ノ カクマク ト モウマク ニ オケル steroid sulfatase ノ ブンプ

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Distribution of steroid sulfatase was studied immunohistochemically as well as enzyme-histochemically in rat cornea and retina. The immunostaining with a monoclonal antibody specific to the enzyme revealed that the enzyme protein was abundantly distributed in inner segments of photoreceptor cells and in retinal pigment epithelial cells. Other parts of the retina contained less amounts of the enzyme. The cornea showed no specific immunostaining. Electron microscopic immunohistochemical studies revealed that the enzyme molecules were localized in endoplasmic reticula and nuclear envelopes of the retinal cells. Distribution patterns resulted from the enzyme-histochemical studies were comparable to the immunohistochemical ones. The enzyme activity, however, was detected not only in these retinal cells, but also in corneal cells, including corneal epithelial, stroma, and endothelial cells. No regional difference on the enzyme activity was shown in the cornea.

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