多血小板血漿(PRP)精製過程における遠心分離についての検討 Investigations into Centrifugal Separations for Preparation of Platelet Rich Plasma (PRP)
Platelet rich plasma (PRP) has beneficial effects on wound healing and bone regeneration. Therefore, PRP is applied in various clinical treatments hereafter, for example, in oral implants and in bone grafts. But the method of centrifugal separation for preparation of PRP is not established, and it varies from each hospital. So, we investigated an efficient one. We prepared PRP by centrifugal separations at various revolutions per minute and various spin times, and measured concentrations of platelets. Concentrations of platelets of PRP separated at higher revolutions with longer spin times are significantly lower than those separated at a moderate number of revolutions with moderate spin times. It is made clear that the most efficient centrifugal separation is at about 1000 rpm, 5 minutes. And it is suggested that a double spin technique is far more efficient than a single spin technique.
奥羽大学歯学誌 31(4), 243-247, 2004-12-30