封入標本からの再染色，ＤＮＡ抽出に関する封入剤の影響

• 池田 聡

  総合病院土浦協同病院病理部

• 木村 博

  総合病院土浦協同病院病理部

• 本間 恵美子

  総合病院土浦協同病院病理部

• 横溝 早苗

  総合病院土浦協同病院病理部

• 船越 尚哉

  総合病院土浦協同病院情報室

書誌事項

タイトル別名

• Influence of mounting media on re-staining of cytology smears and DNA extraction

抄録

Objective: New techniques, such as cell transfer, DNA extraction and the re-staining method, are required for accurate diagnosis in cytology. We investigated the influence of the mounting media in applying the new techniques.<BR>Materials and Methods: Four kinds of mounting media were evaluated. Specimens without mounting media were used as controls. After 4 days mounting, 60 conditions (6 smears×5 media× 2 antigens) were investigated by immuno-staining for alcohol-fixed cytology smears and formalin-fixed paraffin sections. Similar observations were performed after mounting of one month for about 15 other conditions (3 smears × 5 media). We also extracted DNA from specimens under 30 different conditions (6 smears × 5 media) for PCR detection of β globin.<BR>Results: The results indicated that the staining intensity and PCR product were dependent on the kind of mounting media used. One type of mounting medium in particular caused deterioration of the antigen and DNA applicability for PCR.<BR>Conclusion: Mounting media might be a confounding factor in applying new cytological diagnostic techniques.

収録刊行物

• 日本臨床細胞学会雑誌

  日本臨床細胞学会雑誌 45 (2), 73-76, 2006

  公益社団法人　日本臨床細胞学会

キーワード

• Immunocytochemistry
• Restaing method
• DNA extraction
• Mounting media

詳細情報

• CRID: 1390282679673331840
• NII論文ID: 130003926878
• NII論文ID: 110004686497
• NII書誌ID: AN00198721
• DOI: 10.5795/jjscc.45.73
• ISSN: 18827233
• ISSN: 03871193
• ISSN: http://id.crossref.org/issn/03871193
• Web Site: https://search.jamas.or.jp/link/ui/2006174134
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可