Nucleotide Sequence of a Human Genomic DNA Fragment Containing the PCNA Pseudogene and its Localization on Chromosome 4

  • ARIMORI Kaori
    4th Department of Internal Medicine, Tokai University School of Medicine
  • KOIDO Shigeo
    Department of Pathology, Jikei University School of Medicin
  • YOSHIMURA Shinichi
    Department of Molecular Life Science (Cell Biology), Tokai University School of Medicine
  • TANIGUCHI Yasushi
    Department of Molecular Life Science (Cell Biology), Tokai University School of Medicine
  • ARIMORI Shigeru
    4th Department of Internal Medicine, Tokai University School of Medicine
  • MORIUCHI Tetsuya
    Department of Molecular Life Science (Cell Biology), Tokai University School of Medicine

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A one kb human genomic DNA fragment, containing a processed pseudogene of a proliferating cell nuclear antigen (PCNA/DNA polymerase δ auxiliary protein), was isolated and sequenced. The PCNA pseudogene consisted of the 3' half of exon 4 and the 5' half of exon 5 of the PCNA gene, and shared 84% nucleotide homology with the human PCNA cDNA. The PCNA pseudogene was localized on human chromosome 4, based on data obtained from a panel of human-mouse hybrid cell lines.

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