抄録
タマネギ種子をトレハロース,ラフィノースを含む濾紙上に数日間静置することにより,プライミング効果が得られることが示された.この効果は高塩類濃度,高浸透圧および低温条件の不良発芽環境下でも維持された.またタマネギ品種が本来持っている発芽能力の差により,発芽促進程度に違いは見られたが,試験した3品種のいずれにおいてもプライミング効果は認められた.さらにトレハロースによりプライミング処理を行った種子を異なる保存温度条件下で9週間保存した場合でも,処理直後の効果とほぼ同等のプライミング効果が得られる事から,プライミング処理は長期間持続することが明らかになった.以上の結果から,タマネギ種子に対して,トレハロース,ラフィノースがプライミング効果を示すことが明らかとなった.
Onion (Allium cepa L.) seeds were primed for 4-6 days at 15℃ on a filter paper moistened with trehalose or raffinose solutions. These priming treatments accelerated the speed and uniformity of germination, and were comparable to the polyethylene glycol (PEG) treatment, which is used widely as a priming technique. Onion seeds stored at 5, 15 and 22℃ for 9 weeks after priming with trehalose or raffinose still showed high germination speed and uniformity. The priming effect of trehalose was observed in all three onion cultivars tested even though there were differences in the effect based on their original germination abilities. Under stressful conditions such as NaCl (100mM), PEG (10%) or low temperature (15℃), primed seeds treated by trehalose (0.4M) or raffinose (0.4M) showed germination rates as high as 30-50% after 2 days incubation. This is in contrast with germination rates of around 2% in the unprimed seeds. From these results, the trehalose and raffinose treatments are considered useful to improve seed germination ability in onion seeds.
