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Urinary free sulfate was measured after protein intake in order to examine the possibility of biological monitoring using ion chromatography. Urinary free sulfate excretions reached their maximum values in 6 hours after administration in three levels of beef doses. Free sulfate excretions in the urine samples for 16-hour periods and for the first 6 hours after administration were related to the doses beef administered. Free sulfate excretion, adjusted using the sulfate-creatinine ratio, in the urine 6 hours after the administration was related to the dose of beef administered. These experimental results showed that the amount of protein intake could be estimated by urinary free sulfate.
Urinary free sulfate was measured after protein intake in order to examine the possibility of biological monitoring using ion chromatography. Urinary free sulfate excretions reached their maximum values in 6 hours after administration in three levels of beef doses. Free sulfate excretions in the urine samples for 16-hour periods and for the first 6 hours after administration were related to the doses beef administered. Free sulfate excretion, adjusted using the sulfate-creatinine ratio, in the urine 6 hours after the administration was related to the dose of beef administered. These experimental results showed that the amount of protein intake could be estimated by urinary free sulfate.