ヒト歯肉縁下プラーク中のＡｃｔｉｎｏｂａｃｉｌｌｕｓ　ａｃｔｉｎｏｍｙｃｅｔｅｍｃｏｍｉｔａｎｓとその血清型分布

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  • Actinobacillus actinomycetemcomitans in human subgingival plaque and distribution of serotype.

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This study examined serotype distribution of Actinobacillus actinomycetemcomitans in the Japanese periodontal patients.<BR>A total of 35 subjects were included in the study. They were between 16 to 56 years, and demonstrated at least the periodontal pockets with depth greater than 4 mm. Subgingival dental plaque was examined for the presence of Actinobacillus actinomycetemcomitans using previously descrived techniques. Briefly, supragingival dental plaque was removed by means of sterile cotton pledgets. Three sterile paper points were then inserted into the periodontal pocket untill resistance was met and left inplace for 30 seconds. Paper points from each sites were then pooled into tube containing 4.5ml of sterile prereduced Ringer's solution. Those were dispersed for 60 seconds and 0.1ml aliquotes were plated onto a selective medium TBSV) for isolation of Actinobacillus actinomycetemcomitans. After 48-72 hours of incubation at37.. in an anaerobic chambar (Gas-Pak System), the agar plate were examind for Actinobacillus actinomycetemcomitans. Colony morphology was observed directly on TSBV agar. All colony represented rough surface at primaly culture.<BR>Using serotype specific rabbit antisera, oral isolates of Actinobacillus actinomycetemcomitans were assigned to one of three reported serotypes by means of Oucterlony agar-gel diffusion techuniques . The Ouchterlony agar-gel diffusion test was used with two different antigen preparations: sonic extract and autoclave extract. The agglutination test revealed that using autoclave extract of whole celles of Actinobacillus actinomycetemcomians was clearer than that of sonic extract. A total of 158 isolates of Actinobacillus actinomycetemcomitans from oral cavity of 25 subjects were serologically categolized. Sixteen sites exhibited serotype c, whereas 2 sites exhibited serotype a. Seventy three isolates of Actinobacillus actinomycetemcomitans from 14 sites were not serologically characterized. No isolates of Actinobacillus actinomycetemcomitans represented serotype b.<BR>This study indicates that the high prevalence of serotype c in the subgingival plaque of Japanese periodontal patients compared to the much lower prevalence of serotype a and b.

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