リコンビナントヒトＢＭＰ‐２を応用した歯周組織再生療法に関する研究

DOI 被引用文献8件 参考文献32件

書誌事項

タイトル別名
  • A Study of Periodontal Regenerative Therapy Using Recombinant Human BMP-2.

この論文をさがす

抄録

This study was carried out to examine clinical applications of periodontal regeneration therapy using rhBMP-2. Improved fibrous collagen membrane (IFCM 1) and a combination of polylactic acid-polyglycolic acid-copolymer and gelatin sponge (PGS) were used as the rhBMP-2 carrier. The availability of rhBMP-2 and those carriers were evaluated by clinical, radiographic, histopathologic, and histometric observation.<BR>In the 1st experiment, 0.5mg IFCM 1 or 15mm3 PGS were combined with 1, 5 or 10μg rhBMP-2, and implanted on the distobuccal bony plate of canines in cats (N=8). The animals were sacrificed after 3, 5, 7 and 9 weeks and the good combination ratio of rhBMP-2 and each of the carriers were histopathologically examined. The best result were obtained with either 10.0μg rhBMP-2/1.0mg IFCM 1 or 1.0μg rhBMP-2/3.0mm3 PGS.<BR>Following of 1st experiment in the 2nd experiment, materials with the best combination ratio were implanted into artificial classIII furcation bony defect of premolars in cats (N=10). Clinical and standardized radiographic observation were performed at 1, 3, 5, 7, 9 and 12 weeks postsurgically. Healing was generally uneventful without signs of infection. On radiographic observasion increasing radiopacity gradually appeared. The cats sacrificed 6 and 12 weeks postsurgically and then histopathologic and histometric observations were made. Histometric recording included the height of alveolar bone regeneration (NB), the height of cementum regeneration (NC) and the hight of periodontal ligament regeneration (NL). At the rhBMP-2/IFCM 1 site, NB was 2.58mm, NC was 2.50mm, NL was 2.39mm. In rhBMP-2/PGS site, NB was 2.57mm, NC was 2.93mm, NL was 2.62 mm.<BR>These results show that periodontal regeneration with the best combination ratio of rhBMP-2 and each carrier has the potential for clinical application. However, ankylosis was observed in 3 out of 8 teeth in rhBMP-2/IFCM 1 sites and epithelial downgrowth was frequently observed at rhBMP-2/ PGS sites. Further improvements of the carriers are necessary to overcome difficulties encountered in the present experiment.

収録刊行物

被引用文献 (8)*注記

もっと見る

参考文献 (32)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ