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Eostrobilops nipponica reikoae n. subsp. (Strobilopsidae), from Osaka, is described. E. n. reikoae differs from nominate subspecies, E. n. nipponica by its distributional area, smaller size, a stout shell and more darkish red-brown color. Eostrobilops, so far only known from Central Japan, is for the first time recorded to occur in Western Japan.