イヌの骨髄由来樹状細胞の効率的な誘導

• ISOTANI Mayu

  Department of Veterinary Clinical Pathology, Nippon Veterinary and Life Science University

• KATSUMA Kensuke

  Department of Veterinary Clinical Pathology, Nippon Veterinary and Life Science University

• TAMURA Kyoichi

  Department of Veterinary Clinical Pathology, Nippon Veterinary and Life Science University

• YAMADA Misato

  Department of Veterinary Clinical Pathology, Nippon Veterinary and Life Science University

• YAGIHARA Hiroko

  Department of Veterinary Clinical Pathology, Nippon Veterinary and Life Science University

• AZAKAMI Daigo

  Department of Veterinary Clinical Pathology, Nippon Veterinary and Life Science University

• ONO Kenichiro

  Department of Veterinary Clinical Pathobiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo

• WASHIZU Tsukimi

  Department of Veterinary Clinical Pathology, Nippon Veterinary and Life Science University

• BONKOBARA Makoto

  Department of Veterinary Clinical Pathology, Nippon Veterinary and Life Science University

書誌事項

タイトル別名

• Efficient Generation of Canine Bone Marrow-Derived Dendritic Cells

抄録

Because of their unsurpassed potency in presenting antigens to naive T cells, dendritic cells are considered to be an important candidate in the development of immunotherapeutic strategies. Despite the high potential of dendritic cell-based immunotherapy, as a so-called dendritic cell vaccination, few clinical approaches using dendritic cell vaccination have been performed in the dog because of very limited information regarding the generation of canine dendritic cells and their functional properties. We therefore established a protocol for the efficient generation of dendritic cells from canine bone marrow cells using recombinant feline granulocyte-macrophage colony-stimulating factor and canine interleukin-4. Dendritic cells were generated efficiently: a yield of 1-9 × 10⁶ cells per approximately 0.5 ml of canine bone marrow aspiration was achieved. These dendritic cells showed features shared with mouse and human dendritic cells: dendrite morphology, expression of surface markers MHC class II and CD11c, and up-regulation of molecules related to antigen presentation (MHC class II, B7-1, and B7-2) by activation with lipopolysaccharide. Moreover, the dendritic cells demonstrated phagocytic activity, processing activity of pinocytosed proteins, and activation of allogeneic T cells far more potent than that by macrophages. Our findings suggest that the bone marrow-derived dendritic cells are functional for the capturing and processing of antigens and the initiation of T cell responses.<br>

収録刊行物

• Ｔｈｅ　Ｊｏｕｒｎａｌ　ｏｆ　Ｖｅｔｅｒｉｎａｒｙ　Ｍｅｄｉｃａｌ　Ｓｃｉｅｎｃｅ

  Ｔｈｅ　Ｊｏｕｒｎａｌ　ｏｆ　Ｖｅｔｅｒｉｎａｒｙ　Ｍｅｄｉｃａｌ　Ｓｃｉｅｎｃｅ 68 (8), 809-814, 2006

  公益社団法人 日本獣医学会

キーワード

• bone marrow
• canine
• dendritic cell
• generation

詳細情報

• CRID: 1390282681403331840
• NII論文ID: 130000447779
• NII論文ID: 110004814817
• NII書誌ID: AA10796138
• DOI: 10.1292/jvms.68.809
• ISSN: 13477439
• ISSN: 09167250
• NDL書誌ID: 8075377
• Web Site: https://agriknowledge.affrc.go.jp/RN/2010733474
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I8075377
• Web Site: http://www.jstage.jst.go.jp/article/jvms/68/8/68_8_809/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2006277195
• 本文言語コード: en
• データソース種別:
  • JaLC
  • IRDB
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• 抄録ライセンスフラグ: 使用不可