The Delay of Multiplication of East Asian Passiflora Virus-Amami-O-shima Isolate (EAPV-AO) by the Pre-inoculation of EAPV-Ibusuki Isolate (EAPV-IB) The Delay of Multiplication of East Asian Passiflora Virus-Amami-O-shima Isolate (EAPV-AO) by the Pre-inoculation of EAPV-Ibusuki Isolate (EAPV-IB)

The effects of the pre-inoculation of a mild strain of East Asian Passiflora virus (EAPV-IB) on a severe strain of EAPV-AO were investigated. The longer a period between the pre- and challenge inoculation (from 7 days up to 60), the stronger the multiplication of AO was suppressed. Finally, AO was detected in the leaves of the apex and lateral branches, and IB did not provide a complete attenuated virus to prevent AO. However, the delayed effects of IB pre-inoculation against an AO-challenge indicate that IB has the latent ability to be the basic material for a more effective attenuated virus.

The effects of the pre-inoculation of a mild strain of East Asian Passiflora virus (EAPV-IB) on a severe strain of EAPV-AO were investigated. The longer a period between the pre- and challenge inoculation (from 7 days up to 60), the stronger the multiplication of AO was suppressed. Finally, AO was detected in the leaves of the apex and lateral branches, and IB did not provide a complete attenuated virus to prevent AO. However, the delayed effects of IB pre-inoculation against an AO-challenge indicate that IB has the latent ability to be the basic material for a more effective attenuated virus.