フェニルボロン酸修飾アゾ色素の糖応答性に対するポリアリルアミンの効果(<特集>生命科学と分析化学)  [in Japanese] Effects of Poly(allylamine) on the Sugar-Binding Properties of a Phenylboronic Acid-Appended Azo Dye(<Special Article>Bioanalysis and Life Science)  [in Japanese]

A phenylboronic acid-appended azo dye (Azo-PBA) binds saccharides and shows a color change at neutral pH. We investigated the effects of poly (allylamine) (PAA) on the binding property of Azo-PBA. The UV-visible absorption spectra of Azo-PBA were changed in the presence of PAA, depending on the concentrations. This result indicates the formation of Azo-PBA aggregates. In the absence of PAA, the binding constants of Azo-PBA to D-glucose and D-fructose were calculated to be 1.4 and 75M^<-1>, respectively. In the presence of PAA, the binding constant to D-glucose was 130M^<-1>, which was 93-times higher than the original binding constant, while the binding constant to D-fructose was increased only 3.3-times. These results show that the coexistence of PAA can selectively enhance the binding ability of Azo-PBA to D-glucose. However, it was found that a small amount of D-fructose interfered in the measurement of D-glucose.