ダイアンサス属野生種Dianthus capitatus ssp. andrzejowskianus由来の劣性一重咲き遺伝子に連鎖したDNAマーカー(育種・遺伝資源)  [in Japanese] DNA Markers Linked to a Recessive Gene Controlling Single Flower Type Derived from a Wild Species, Dianthus capitatus ssp. andrzejowskianus(Breeding & Germplasm Resource)  [in Japanese]

一重咲き,八重咲きの花型はカーネーションの重要形質の一つである.カーネーション連鎖地図作成に用いた集団を材料に,バルク法により一重咲き,八重咲きの形質に関与するRAPDマーカーを探索した.その結果,野生種Dianthus capitatus ssp. andrzejowskianus由来の劣性一重咲き遺伝子と連鎖する4つのRAPDマーカーが見いだされた.特に,同一のバンドパターンを示したOM19-800,AT90-1000,DT52-700は,一重の45個体で存在し八重の82個体で存在せず,一重八重の形質とマーカーバンドの有無が完全に一致し,一重咲き遺伝子に密に連鎖することが示された.これらのうちAT90-1000のSTS化に成功した.連鎖解析の結果,この一重咲き遺伝子は作成したカーネーション連鎖地図において第16連鎖群に座上することがわかった.得られたSTSマーカーの汎用性を検証するため,一重のカーネーション4品種からDNAを抽出してマーカーの有無を調べたが,4品種ともマーカーは存在せず,本分離集団以外への汎用性は認められなかった.

Flower type of single or double is one of the important characters in carnation (Dianthus caryophyllus L.). Therefore, we selected random amplified polymorphic DNA (RAPD) markers associated with the genes controlling flower type in a segregated population of 127 progeny plants derived from a cross between 'Carnation Nou No. 1' (double) and 'Pretty Favvare' (single), which were used for construction of our genetic linkage map in carnations. Four RAPD markers identified by bulked segregant analysis of 696 primers were linked to a recessive gene controlling a single flower type derived from a wild species, D. capitatus ssp. andrzejowskianus. In particular, three RAPD markers, OM19-800, AT90-1000, DT52-700 appeared in all 45 single plants individually, but not in any of the double plants, indicating these three markers were tightly linked to the gene controlling the single flower type. The RAPD marker AT90-1000 was successfully converted to a sequence-tagged site (STS) marker. Linkage analysis revealed that this single gene was located on group 16 in our genetic linkage map. However, the STS marker was not detected in the four single flowered carnation cultivars. This result indicates that this STS marker is highly specific for the single gene derived from D. capitatus ssp. andrzejowskianus.