Vascular Invasion of Epiphyseal Growth Plate in Osteopetrotic (op/op) Mouse Tibiae
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- Sugiura Junko
- Department of Oral Medical Sciences, Division of Oral Pathology, Ohu University School of Dentistry
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- Ito Hiroshi
- Department of Oral Medical Sciences, Division of Oral Pathology, Ohu University School of Dentistry
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- Sakurai Yuuko
- Department of Oral Medical Sciences, Division of Oral Pathology, Ohu University School of Dentistry
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- Okuyama Noriko
- Department of Oral Medical Sciences, Division of Oral Pathology, Ohu University School of Dentistry
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- Yamasaki Akira
- Department of Oral Medical Sciences, Division of Oral Pathology, Ohu University School of Dentistry
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Abstract
To clarify what type of cells lead vascular invasion of epiphyseal growth plate in developing long bones, we conducted immunohistochemical and electron microscopic studies on the op/op mouse tibia which has an inheriting deficiency of macrophages and osteoclasts. Despite an absence of both TRAP-positive osteoclasts and F4/80-positive macrophages, resorption of epiphyseal cartilage followed by vascular invasion was evident in op/op mouse tibiae. Electron microscopic observation revealed that cells subjacent to the lowermost hypertrophic chondrocyte lacunae were almost exclusively vascular endothelial cells. Immunohistochemically, both cellular elements and extracellular matrix at the vascular invasion front of op/op mouse epiphysis were strongly positive for MMP-9. In situ hybridization revealed a distinct localization of mRNA for MMP-9 in cells located at the same region. From these findings, we hypothesize that vascular endothelial cells themselves are primarily responsible for resorbing the transverse septa of hypertrophic chondrocytes lacunae, and neither osteoclasts nor macrophages involve in this process.
Journal
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- Journal of Hard Tissue Biology
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Journal of Hard Tissue Biology 15 (3), 96-100, 2006
THE SOCIETY FOR HARD TISSUE REGENERATIVE BIOLOGY
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Details 詳細情報について
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- CRID
- 1390282679435780608
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- NII Article ID
- 130004480451
- 110006151629
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- NII Book ID
- AA11074332
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- ISSN
- 1880828X
- 13417649
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- Text Lang
- en
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- Data Source
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- JaLC
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed