Molecular Authentication of Radix Puerariae Lobatae and Radix Puerariae Thomsonii by ITS and 5S rRNA Spacer Sequencing

• Sun Ye

  Department of Biochemistry and Institute of Chinese Medicine, The Chinese University of Hong Kong South China Botanical Garden, The Chinese Academy of Sciences

• Shaw Pang-Chui

  Department of Biochemistry and Institute of Chinese Medicine, The Chinese University of Hong Kong

• Fung Kwok-Pui

  Department of Biochemistry and Institute of Chinese Medicine, The Chinese University of Hong Kong

抄録

In the present study, we examined nuclear DNA sequences in an attempt to reveal the relationships between Pueraria lobata (WILLD). OHWI, P. thomsonii BENTH., and P. montana (LOUR.) MERR. We found that internal transcribed spacer (ITS) sequences of nuclear ribosomal DNA are highly divergent in P. lobata and P. thomsonii, and four types of ITS with different length are found in the two species. On the other hand, DNA sequences of 5S rRNA gene spacer are highly conserved across multiple copies in P. lobata and P. thomsonii, they could be used to identify P. lobata, P. thomsonii, and P. montana of this complex, and may serve as a useful tool in medical authentication of Radix Puerariae Lobatae and Radix Puerariae Thomsonii.

収録刊行物

• Ｂｉｏｌｏｇｉｃａｌ　＆　Ｐｈａｒｍａｃｅｕｔｉｃａｌ　Ｂｕｌｌｅｔｉｎ

  Ｂｉｏｌｏｇｉｃａｌ　＆　Ｐｈａｒｍａｃｅｕｔｉｃａｌ　Ｂｕｌｌｅｔｉｎ 30 (1), 173-175, 2007

  公益社団法人 日本薬学会

キーワード

• 5S rRNA gene spacer
• internal transcribed spacer
• <i>Pueraria lobata</i>
• <i>Pueraria montana</i>
• <i>Pueraria thomsonii</i>

詳細情報

• CRID: 1390001204626963200
• NII論文ID: 110006153288
• NII書誌ID: AA10885497
• DOI: 10.1248/bpb.30.173
• ISSN: 13475215
• ISSN: 09186158
• NDL書誌ID: 8590437
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I8590437
• Web Site: http://www.jstage.jst.go.jp/article/bpb/30/1/30_1_173/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2007175146
• 本文言語コード: en
• データソース種別:
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  • NDL
  • Crossref
  • CiNii Articles
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