ファージディスプレイによる診断治療に有用なヒト型抗体医薬の開発

• 伊藤 邦彦

  静岡県立大学薬学部臨床薬効解析学分野

書誌事項

タイトル別名

• Development of Diagnostically and Therapeutically Useful Human Antibody Medicines by Phage Display System
• 誌上シンポジウム ファージディスプレイによる診断治療に有用なヒト型抗体医薬の開発
• シジョウ シンポジウム ファージディスプレイ ニ ヨル シンダン チリョウ ニ ユウヨウ ナ ヒトガタ コウタイ イヤク ノ カイハツ

抄録

  Phage display has been utilized for making recombinant antibody fragments (Fab or single chain Fv) of human, mouse, or other origins. After construction of an antibody combinatorial library, antigen-specific recombinant antibody fragments can be easily isolated by biopanning of the phage library displaying antibody fragment fused with viral coat protein III against antigen proteins, antigen-expressing live cells, or fixed cells. Using this technique, a variety of human recombinant antibody fragments can be retrieved from bone marrow cells, lymph node cells, or peripheral blood cells of patients with infectious diseases, autoimmune diseases, and cancer. To develop diagnostically and therapeutically useful human antibody medicines, we should first select recombinant antibody fragments not only with antigen-binding activity but also with bioactivity such as virus or toxin neutralization, or tumor-specific cytotoxicity. To achieve this goal, several steps in antibody phage display may be improved: 1) a larger library should be constructed for possible isolation of minor populations present in the repertoire; 2) the biopanning procedure should be improved for isolation of antibody fragments reactive with immunologically minor epitopes; 3) the screening procedure should be based on the measurement of the bioactivity as well as the antigen-binding activity; 4) if necessary, the affinity and specificity of selected antibody fragments should be improved. In this review, I discuss how to isolate clinically useful recombinant antibody fragments efficiently using a phage display system introducing our achievements.<br>

収録刊行物

• 薬学雑誌

  薬学雑誌 127 (1), 43-53, 2007-01-01

  公益社団法人 日本薬学会

キーワード

• phage display
• human antibody medicine
• diagnosis
• therapy

詳細情報

• CRID: 1390001206129616256
• NII論文ID: 110006155009
• NII書誌ID: AN00284903
• DOI: 10.1248/yakushi.127.43
• ISSN: 13475231
• ISSN: 00316903
• NDL書誌ID: 8631869
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I8631869
• Web Site: https://www.jstage.jst.go.jp/article/yakushi/127/1/127_1_43/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2007160417
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• 抄録ライセンスフラグ: 使用不可