Kinetic Study on the Degradation of Meclophenoxate Hydrochloride in Alkaline Aqueous Solutions by High Performance Liquid Chromatography
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- El-BARDICY Mohammad Galal
- Department of Analytical Chemistry, Faculty of Pharmacy, Cairo University
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- LOTFY Hayam Mahmoud
- Department of Analytical Chemistry, Faculty of Pharmacy, Cairo University
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- El-SAYED Mohammad Abdalla
- Department of Analytical Chemistry, Faculty of Pharmacy, Cairo University
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- El-TARRAS Mohammad Fayez
- Department of Analytical Chemistry, Faculty of Pharmacy, Cairo University
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Abstract
A high performance liquid chromatographic method was developed and validated for determination of meclophenoxate hydrochloride (I) in the presence of its degradation product (p-chlorophenoxy acetic acid) (II). Separation of (I) from (II) was performed using a ZORBAX ODS column with a mobile phase consisting of 0.2% triethylamine in 0.01 M ammonium carbonate: acetonitrile (70:30 v/v). The method showed high sensitivity with good linearity over the concentration range of 50 to 400 μg/ml. The method was successfully applied to the analysis of a pharmaceutical formulation containing (I) with excellent recovery. A kinetics investigation of the alkaline hydrolysis of (I) was carried out in sodium hydroxide solutions of 1, 1.5 and 2 N by monitoring the parent compound itself. The reaction order of (I) followed pseudo-first order kinetics. The activation energy could be estimated from the Arrhenius plot and it was found to be 12.331 kcal/mole.<br>
Journal
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- YAKUGAKU ZASSHI
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YAKUGAKU ZASSHI 127 (1), 193-199, 2007
The Pharmaceutical Society of Japan
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Keywords
Details 詳細情報について
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- CRID
- 1390001206129559808
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- NII Article ID
- 110006155025
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- NII Book ID
- AN00284903
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- ISSN
- 13475231
- 00316903
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- NDL BIB ID
- 8632276
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed