Experimental hybridization among Oryzias species. 2. karyogamy and abnormality of chromosome separation in the cleavage of interspecific hybrids between Oryzias latipes and O. javanicus
書誌事項
- タイトル別名
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- Experimental Hybridization among Oryzias Species. II. Karyogamy and Abnormality of Chromosome Separation in the Cleavage of Interspecific Hybrids between Oryzias latipes and O. javanicus
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In interspecific hybridization between Oryzias latipes and O. javanicus, all hybrid embryos failed to develop and died before hatching. Cytological examination of fertilization and early development was performed to discover the cause of lethal development. When O. latipes eggs were inseminated by sperm of O. javanicus, the cortical reaction was induced normally. Chromosomal material in the fertilized eggs was visualized using the DNA-specific fluorochrome Hoechst. The spermatozoon was capable of penetrating into the egg cytoplasm through the micropyle, and the sperm nucleus transformed to the male pronucleus. The female pronucleus that formed after extrusion of the second polar body migrated towards the male pronucleus. The female and the male pronuclei underwent DNA synthesis and encountered each other in the center of the blastodisc, fused with one another and formed a zygote nucleus before breakdown of the nuclear envelope. Metaphase chromosomes with electron dense chromatin regions were abnormally divided into each blastomere in cleavage. The abnormally separating chromatin masses were also labeled by BrdU. The abnormal separation resulting in partial loss of fragmented chromatin might be a cause of abortive development in the interspecific hybrids between O. latipes and O. javanicus.
収録刊行物
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- Zoological Science
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Zoological Science 20 (11), 1381-1387, 2003-11
日本動物学会
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詳細情報 詳細情報について
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- CRID
- 1050001338995322112
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- NII論文ID
- 110006162823
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- NII書誌ID
- AA10545874
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- HANDLE
- 2115/32966
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- NDL書誌ID
- 6768533
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- ISSN
- 02890003
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- 本文言語コード
- en
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- 資料種別
- journal article
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- データソース種別
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- IRDB
- NDL
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- NDL-Digital
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