ゲル被覆サギソウプロトコーム設置法により育成した自生地におけるサギソウ球根の生育評価(繁殖・育種)  [in Japanese] Growth Assay of Daughter Tubers from the Tubers of Habenaria radiata (Thunb.) K. Spreng. Seedlings Gel Covered and Inoculated with Orchid Mycorrhizal Fungi in Habitat(Propagation & Transplant Production)  [in Japanese]

自生地で菌根菌接種ゲル被覆サギソウプロトコーム由来の実生個体に形成された球根から翌年も肥大した新球根が形成され,やがて発蕾・開花に至る可能性があるかどうか,また,自生地に生息する菌根菌にはサギソウ球根形成促進効果があるかどうかを検討した.自生地に植え付けた球根から新たに形成された球根は約2倍に肥大した.サギソウの球根垂が150mg以上に肥大すると,半数が発蕾・開花することから,自生地で菌根菌接種ゲル被覆サギソウプロトコーム由来の実生苗から形成された球根は,5年後に発蕾・開花する可能性があると考えられた.自生地に生息する菌根菌にはサギソウの球根形成を促進する菌株も認められたので,その菌株をゲル被覆サギソウプロトコームに接種して自生地に設置することが望ましいと考えられた.自生地で形成される新球根をさらに肥大させるには,自生地の環境条件も検討する必要がある.

It was investigated whether the daughter tubers formed from tubers that were formed from the protocorms of Habenaria radiata gel covered and inoculated with orchid mycorrhizal fungi grew larger than the tubers formed from seedlings, and whether these daughter tubers had the possibility of forming flower buds or reaching bloom in habitat. Whether the orchid mycorrhizal fungi in habitat was effective in promoting larger tuber formation. It was considered that half of tubers could form flower buds or reached bloom when the tuber weight of H. radiata enlarged beyond 150mg. The daughter tubers grew larger than the tubers formed from seedlings, and the growth rate of the daughter tubers in habitat were two-fold larger. Therefore, the daughter tubers were thought to have the possibility of forming flower buds or reaching bloom after about five years. Because orchid mycorrhizal fungi which promotes tuber formation of H. radiata was recognized in habitat, it is considered desirable to inoculate this fungi on gel covered protocorms. Further, it was thought that environmental conditions must be investigated in order to enlarge the daughter tuber in habitat by methods in addition to the growth promoting effect of mycorrhiza which also lives in habitat.