Nanomaterials Induce Oxidized Low-Density Lipoprotein Cellular Uptake in Macrophages and Platelet Aggregation

    • Niwa Yasuharu
    • Department of Epidemiology, Research Institute, National Cardiovascular Center
    • Iwai Naoharu
    • Department of Epidemiology, Research Institute, National Cardiovascular Center

Access this Article

  • CiNii Fulltext PDF

    Open Access

  • J-STAGE

  • CrossRef

Search this Article

Abstract

Background Nanomaterials have numerous potential benefits for society, but the potential hazards of nanomaterials on human health are poorly understood. Nanomaterials are known to pass into the circulatory system in humans, causing vascular injuries that might play a role in the development of atherosclerosis. The present study aimed to determine the effects of chronic exposure to nanomaterials on macrophage phenotype and platelet aggregation. Methods and Results Cultured macrophages(RAW264.7) were treated with carbon black(CB) and water-soluble fullerene(C_<60>(OH)_<24>) from 7 to 50 days. Individually, CB had no significant effects on RAW264.7 cell growth, whereas C_<60>(OH)_24 alone or CB and C_<60>(OH)_24 together with oxidized low-density lipoprotein(Ox-LDL)(100μg/ml) induced cytotoxic morphological changes, such as Ox-LDL uptake-induced foam cell-like formation and decreased cell growth, in a dose-dependent manner. C_<60>(OH)_24 induced LOX-1 protein expression, pro-matrix metalloprotease-9 protein secretion, and tissue factor mRNA expression in lipid-laden macrophages. Although CB or C_<60>(OH)_24 alone did not induce platelet aggregation, C_<60>(OH)_24 facilitated adenosine diphosphate(ADP)-induced platelet aggregation. Furthermore, C_<60>(OH)_24 acted as a competitive inhibitor of ADP receptor antagonists in ADP-mediated platelet aggregation. Conclusions The present study confirmed novel effects of nanomaterials in macrophages and platelets. These effects suggest that exposure to nanomaterials might be a risk for atherothrombotic diseases.

Journal

Circulation journal : official journal of the Japanese Circulation Society   [List of Volumes]

Circulation journal : official journal of the Japanese Circulation Society 71(3), 437-444, 2007-02-20  [Table of Contents]

Japanese Circulation Society

References:  32

You must have a user ID to see the references.If you already have a user ID, please click "Login" to access the info.New users can click "Sign Up" to register for an user ID.

Cited by:  3

You must have a user ID to see the cited references.If you already have a user ID, please click "Login" to access the info.New users can click "Sign Up" to register for an user ID.

Codes

  • NII Article ID (NAID) :
    110006224217
  • NII NACSIS-CAT ID (NCID) :
    AA11591968
  • Text Lang :
    RUS
  • Article Type :
    Journal Article
  • ISSN :
    13469843
  • Databases :
    CJP  CJPref  NII-ELS  J-STAGE