Histochemistry. X. : Distributions of Aluminum, Phosphorus, and Other Elements in Bupleurum falcatum Root Cultivated in Japan(Pharmacognosy,Chemical)
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The concentrations and distributions of nine elements and three saikosaponins in Bupleurum falcatum root cultivated in Japan were histochemically examined by means of an electron microprobe X-ray microanalyzer (EXMA), inductively coupled plasma spectrometry (ICP) and high-performance liquid chromatography. Point and line analyses by semi-quantitative EXMA coupled with quantitative ICP proved to be a convenient and selective method for histochemical analysis of the tissue-specific distributions of the elements in plant roots. The present histochemical examination showed that aluminum and manganese along with three saikosaponins a, c and d occur exclusively in the outermost peripheral layer, consisting of a cork layer and pericycle, of the root, and that phosphorus occurs in the innermost xylem layer at a high concentration.