An Unambiguous Assignment and Structural Analysis Using Solution NMR Experiments of O-Antigen from Escherichia coli ATCC23505 (Serotype O9)

    • TADA Rui
    • Laboratory far Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pharmacy and Life Science
    • NAGI-MIURA Noriko
    • Laboratory far Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pharmacy and Life Science
    • ADACHI Yoshiyuki
    • Laboratory far Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pharmacy and Life Science
    • OHNO Naohito
    • Laboratory far Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pharmacy and Life Science

Abstract

Bacterial lipopolysaccharide from Escherichia coli O9 (O9 LPS) has various characteristic biological activities other than endotoxic activities. The biological activities exhibited depend on the structure of the O-antigen. The O-antigen region of O9 LPS is composed of the mannose homopolysaccharide (MHP). This structure was reported previously, but not all its proton and carbon signals were assigned. In the present study, we completely as sign all proton and carbon signals of the O-antigen of O9 LPS using ^1H- and ^<13>C-NMR spectroscopy, including the DQF-COSY, TOCSY, NOESY, HSQC, H2BC, HSQC-TOCSY and HMBC methods.

Journal

Chemical & pharmaceutical bulletin   [List of Volumes]

Chemical & pharmaceutical bulletin 55(7), 992-995, 2007-07-01  [Table of Contents]

The Pharmaceutical Society of Japan

References:  17

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Codes

  • NII Article ID (NAID) :
    110006317159
  • NII NACSIS-CAT ID (NCID) :
    AA00602100
  • Text Lang :
    ENG
  • Article Type :
    ART
  • ISSN :
    00092363
  • NDL Article ID :
    8783436
  • NDL Source Classification :
    ZS51(科学技術--薬学) // ZP1(科学技術--化学・化学工業)
  • NDL Call No. :
    Z53-D167
  • DOI :
    10.1248/cpb.55.992
  • Databases :
    CJP  NDL  NII-ELS  J-STAGE