微生物酵素の生化学的及び構造生物学的研究と医療への応用

• 芳本 忠

  長崎大学大学院医歯薬学総合研究科薬学系薬品生物工学

書誌事項

タイトル別名

• Biochemistry and Structural Biology of Microbial Enzymes and their Medical Applications
• ビセイブツ コウソ ノ セイカガクテキ オヨビ コウゾウ セイブツガクテキ ケンキュウ ト イリョウ エノ オウヨウ

抄録

  Microbial enzymes were studied from two medicinal viewpoints. First, we examined proline-specific peptidases from pathogenic microorganisms. We found several proline-specific peptidases in pathogenic bacteria. Among them, prolyl tripeptidyl aminopeptidase from Porphylomonas gingivals and prolyl aminopeptidase from Serratia marcescens were crystallized. The complex structures of those enzymes and inhibitors were clarified in X-ray crystallography. Aminopeptidase N, which has wide specificity for amino acids, was distributed in the pathogens. The crystal structure of the aminopeptidase N elucidated the reasons for its wide substrate specificity but inertness to the X-Pro bond. It was also revealed that proline-specific peptidases and aminopeptidase N cooperatively degrade collagen for the uptake of amino acids as nutrition when these bacteria infect cells. Second, we applied enzymes from microorganisms to diagnostic analyses. We found a series of creatinine-metabolizing enzymes in Pseudomonas putida. Creatininase, creatinase, and sarcosine oxidase were coupled and have been developed for a diagnostic analysis kit that examines renal function. The structures of the native and the Mn²⁺-activated creatininases were determined in X-ray crystallography. Based on the structure, the activated enzyme was used for an improved assay kit. The structure of D-3-hydroxybutyrate dehydrogenase from Pseudomonas fragi was also clarified in crystallography. The enzyme is useful for diagnostic analysis of diabetes mellitus while monitoring ketone bodies.<br>

収録刊行物

• 薬学雑誌

  薬学雑誌 127 (7), 1035-1045, 2007-07-01

  公益社団法人 日本薬学会

キーワード

• structural biology
• proline-specific peptidase
• microbial enzyme
• diagnostic analysis

詳細情報

• CRID: 1390282681104737536
• NII論文ID: 110006318151
• NII書誌ID: AN00284903
• DOI: 10.1248/yakushi.127.1035
• COI: 1:STN:280:DC%2BD2szos1ertg%3D%3D
• ISSN: 13475231
• ISSN: 00316903
• HANDLE: 10069/14912
• NDL書誌ID: 8881736
• PubMed: 17603262
• Web Site: https://nagasaki-u.repo.nii.ac.jp/records/21028
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I8881736
• Web Site: https://www.jstage.jst.go.jp/article/yakushi/127/7/127_7_1035/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2007334812
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • IRDB
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• 抄録ライセンスフラグ: 使用不可