Formaldehyde Production by Cells of a Mutant of Candida boidinii S2 Grown in Methanol-limited Chemostat Culture(Microbiology & Fermentation Industry)

    • SAKAI Yasuyoshi
    • Department of Agricultural Chemistry, Faculty of Agriculture, Kyoto University
    • TANI Yoshiki
    • Research Center for Cell and Tissue Culture, Kyoto University

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Abstract

Formaldehyde production was investigated with cells of a mutant, AOU-l, of a methanol yeast, Candida boidinii S2 grown in methanol-limited chemostat culture. The highest productivity was shown with cells from the culture at a dilution rate of 0.075 hr^<-1>, when cells had the highest activity of alcohol oxidase and almost minimum activity of formaldehyde dehydrogenase. Under optimal reaction conditions, 950mM formaldehyde was produced in 10-hr reaction with the cells. By the chemostat culture, not only formaldehyde productivity but also cell productivity was improved in comparison with batch culture. A maximum cell productivity of 0.2g・liter^<-1>・hr^<-1> and a cell yield of 47% were obtained.

Journal

Agricultural and biological chemistry   [List of Volumes]

Agricultural and biological chemistry 50(10), 2615-2620, 1986-10-23  [Table of Contents]

Japan Society for Bioscience, Biotechnology, and Agrochemistry

Codes

  • NII Article ID (NAID) :
    110006322737
  • NII NACSIS-CAT ID (NCID) :
    AA00515312
  • Text Lang :
    ENG
  • ISSN :
    00021369
  • Databases :
    NII-ELS  J-STAGE