Formaldehyde Production by Cells of a Mutant of Candida boidinii S2 Grown in Methanol-limited Chemostat Culture(Microbiology & Fermentation Industry)

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著者

    • SAKAI Yasuyoshi
    • Department of Agricultural Chemistry, Faculty of Agriculture, Kyoto University
    • TANI Yoshiki
    • Research Center for Cell and Tissue Culture, Kyoto University

抄録

Formaldehyde production was investigated with cells of a mutant, AOU-l, of a methanol yeast, Candida boidinii S2 grown in methanol-limited chemostat culture. The highest productivity was shown with cells from the culture at a dilution rate of 0.075 hr^<-1>, when cells had the highest activity of alcohol oxidase and almost minimum activity of formaldehyde dehydrogenase. Under optimal reaction conditions, 950mM formaldehyde was produced in 10-hr reaction with the cells. By the chemostat culture, not only formaldehyde productivity but also cell productivity was improved in comparison with batch culture. A maximum cell productivity of 0.2g・liter^<-1>・hr^<-1> and a cell yield of 47% were obtained.

収録刊行物

  • Agricultural and Biological Chemistry   [巻号一覧]

    Agricultural and Biological Chemistry 50(10), 2615-2620, 1986-10-23  [この号の目次]

    社団法人日本農芸化学会

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