Formaldehyde production by cells of a mutant of Candida boidinii S2 grown in methanol-limited chemostat culture.

• SAKAI Yasuyoshi

  Department of Agricultural Chemistry, Faculty of Agriculture, Kyoto University

• TANI Yoshiki

  Research Center for Cell and Tissue Culture, Kyoto University

Abstract

Formaldehyde production was investigated with cells of a mutant, AOU-1, of a methanol yeast, Candida boidinii S2 grown in methanol-limited chemostat culture. The highest productivity was shown with cells from the culture at a dilution rate of 0.075 hr^-1, when cells had the highest activity of alcohol oxidase and almost minimum activity of formaldehyde dehydrogenase. Under optimal reaction conditions, 950 mM formaldehyde was produced in 10-hr reaction with the cells. By the chemostat culture, not only formaldehyde productivity but also cell productivity was improved in comparison with batch culture. A maximum cell productivity of 0.2 g•liter^-1•hr^-1 and a cell yield of 47% were obtained.

Journal

• Agricultural and Biological Chemistry

  Agricultural and Biological Chemistry 50 (10), 2615-2620, 1986

  Japan Society for Bioscience, Biotechnology, and Agrochemistry

Keywords

• General Agricultural and Biological Sciences
• General Biochemistry, Genetics and Molecular Biology

Details

• CRID: 1390282681443822848
• NII Article ID: 110006322737
• NII Book ID: AA00515312
• DOI: 10.1271/bbb1961.50.2615
• COI: 1:CAS:528:DyaL28Xmt1Cmtr8%3D
• ISSN: 18811280
• ISSN: 00021369
• Text Lang: en
• Data Source:
  • JaLC
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed