Insecticidal Activity of Bipyramidal and Cuboidal Inclusions of δ-Endotoxin and Distribution of Their Antigens among Various Strains of Bacillus thuringiensis(Biological Chemistry)

    • SAMASANTI Wiwit
    • Institute of Biological Control, Faculty of Agriculture, Kyushu University:(Present office)Department of Microbiology, Faculty of Science, Mahidol University
    • TOJO Akihiko
    • Institute of Biological Control, Faculty of Agriculture, Kyushu University
    • AIZAWA Keio
    • Institute of Biological Control, Faculty of Agriculture, Kyushu University

Abstract

Bacillus thuringiensis kurstaki strain HD-1 produces two morphologically different parasporal inclusions of δ-endotoxin, bipyramidal and cuboidal inclusions. We found that the bipyramidal inclusion was composed of a 130,000-dalton subunit protein and was toxic to the silkworm, Bombyx mori (a lepidopteran insect), but not to the mosquito, Aedes aegypti (a dipteran insect) and that the cuboidal inclusion was composed of a 65,000-dalton subunit protein and was associated with the mosquitocidal activity. The distribution of the two proteins, the toxic fragment with a molecular weight of about 60,000 derived from the bipyramidal inclusions and the cuboidal toxin, was studied in various strains of B. thuringiensis by an enzyme-linked immunosorbent assay.

Journal

Agricultural and biological chemistry   [List of Volumes]

Agricultural and biological chemistry 50(7), 1731-1735, 1986-07-23  [Table of Contents]

Japan Society for Bioscience, Biotechnology, and Agrochemistry

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Codes

  • NII Article ID (NAID) :
    110006322972
  • NII NACSIS-CAT ID (NCID) :
    AA00515312
  • Text Lang :
    ENG
  • ISSN :
    00021369
  • Databases :
    NII-ELS  Journal@rchive 

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