Human-human hybridomas secreting IgM-like immunoglobulin with .ALPHA. and .MU. heavy chains.

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著者

    • MURAKAMI Hiroki
    • Institute of Food Science and Technology, Faculty of Agriculture, Kyushu University
    • SHINOHARA Kazuki
    • Institute of Food Science and Technology, Faculty of Agriculture, Kyushu University
    • OMURA Hirohisa
    • Institute of Food Science and Technology, Faculty of Agriculture, Kyushu University

抄録

Human-human hybridomas were produced by fusion of a malignant 6-thioguanine resistant human B lymphoblastoid cell line and normal lymphocytes. The fusion efficiency with lymphocytes from peripheral blood was elevated by preculture of the lymphocytes in the presence of lipopolysaccharides. However, the preculture did not enhance the efficiency with lymphocytes from lung cancer tissue. The resulting hybridomas secreted mostly IgG and/or IgM. A small number of hybridomas secreted IgA. Two IgA secreting hybridomas, 9P-13-4 and 9L-27-9 also produced IgM-like immunoglobulin even after repeated cloning, indicating these hybridomas secrete IgA and IgM-like immunoglobulins simultaneously. The IgM-like immunoglobulin has a molecular weight of 900 K and contains 3 monomeric IgMs and 2 IgAs. This indicates that it is therefore possible to produce a IgM-like immunoglobulin binding to two different antigen determinants, by hybridoma technology.

収録刊行物

  • Agricultural and Biological Chemistry

    Agricultural and Biological Chemistry 50(9), 2217-2223, 1986

    社団法人日本農芸化学会

被引用文献:  2件中 1-2件 を表示

各種コード

  • NII論文ID(NAID)
    110006323056
  • NII書誌ID(NCID)
    AA00515312
  • 本文言語コード
    EN
  • 資料種別
    雑誌論文
  • ISSN
    0002-1369
  • データ提供元
    CJP引用  NII-ELS  J-STAGE 
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