Human-Human Hybridomas Secreting IgM-like Immunoglobulin with α and μ Heavy Chains(Biological Chemistry)

    • MURAKAMI Hiroki
    • Institute of Food Science and Technology, Faculty of Agriculture, Kyushu University

    • SHINOHARA Kazuki
    • Institute of Food Science and Technology, Faculty of Agriculture, Kyushu University
    • OMURA Hirohisa
    • Institute of Food Science and Technology, Faculty of Agriculture, Kyushu University

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Abstract

Human-human hybridomas were produced by fusion of a malignant 6-thioguanine resistant human B lymphoblastoid cell line and normal lymphocytes. The fusion efficiency with lymphocytes from peripheral blood was elevated by preculture of the lymphocytes in the presence of lipopolysaccharides. However, the preculture did not enhance the efficiency with lymphocytes from lung cancer tissue. The resulting hybridomas secreted mostly IgG and/or IgM. A small number of hybridomas secreted IgA. Two IgA secreting hybridomas, 9P-13-4 and 9L-27-9 also produced IgM-like immunoglobulin even after repeated cloning, indicating these hybridomas secrete IgA and IgM-like immunoglobulins simultaneously. The IgM-like immunoglobulin has a molecular weight of 900K and contains 3 monomeric IgMs and 2 IgAs. This indicates that it is therefore possible to produce a IgM-like immunoglobulin binding to two different antigen determinants, by hybridoma technology.

Journal

Agricultural and biological chemistry   [List of Volumes]

Agricultural and biological chemistry 50(9), 2217-2223, 1986-09-23  [Table of Contents]

Japan Society for Bioscience, Biotechnology, and Agrochemistry

Cited by:  2

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Codes

  • NII Article ID (NAID) :
    110006323056
  • NII NACSIS-CAT ID (NCID) :
    AA00515312
  • Text Lang :
    ENG
  • Article Type :
    Journal Article
  • ISSN :
    00021369
  • Databases :
    CJPref  NII-ELS  J-STAGE