Isolation of a complex of endo-cellulases from the gastric teeth of Dolabella auricularia by affinity chromatography.

• ANZAI Hirosi

  Department of Fisheries, College of Agriculture and Veterinary Medicine, Nihon University

• UCHIDA Naoyuki

  Department of Fisheries, College of Agriculture and Veterinary Medicine, Nihon University

• NISHIDE Eiichi

  Department of Fisheries, College of Agriculture and Veterinary Medicine, Nihon University

• NISIZAWA Kazutosi

  Department of Fisheries, College of Agriculture and Veterinary Medicine, Nihon University

• MATSUDA Kazuo

  the Department of Agricultural Chemistry, Faculty of Agriculture, Tohoku University

抄録

Cellooligomer-(EPOX)-Sepharose CL-2B affinity chromatography was used to further purify the main endo-cellulase fraction, E₃-II, obtained from the gastric teeth of the marine mollusc Dolabella auricularia. The cellulase component in E₃-II, which had a strong affinity for the adsorbent in the presence of 1.0 M NaCl, was effectively removed from the non-cellulase protein and separated into two fractions, one (E₃-II-N) with a low affinity and the other (E₃-II-A) with a high affinity for the adsorbent. E₃-II-A, the main component of E₃-II, was desorbed with a buffer solution without NaCl or by the addition of 10mM cellotriitol to the eluent with or without 1.0M NaCl, and it was purified 23.1-fold over the starting material. Fraction A, obtained by further purification of E₃-II-A by gel filtration, was a single protein band on SDS-PAGE (M.W.: 51, 000), and separated into seven protein bands on DISC-PAGE. At least six of the protein bands had individually been demonstrated to be the same molecular weight (M.W.: 31, 000) by Hedrick-Smith plots on DISC-PAGE as by gel filtration. Fraction A was most active at pH 6.3 and it had a typical endo-hydrolysis pattern on CMC.

収録刊行物

• Agricultural and Biological Chemistry

  Agricultural and Biological Chemistry 52 (3), 633-640, 1988

  公益社団法人 日本農芸化学会

キーワード

• General Agricultural and Biological Sciences
• General Biochemistry, Genetics and Molecular Biology

詳細情報

• CRID: 1390001206463806464
• NII論文ID: 110006323689
• NII書誌ID: AA00515312
• DOI: 10.1271/bbb1961.52.633
• ISSN: 18811280
• ISSN: 00021369
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可