Purification and characterization of endo-α-N-acetyl-galactosaminidase from Alcaligenes sp.

• FAN Jian-Qiang

  Kacho College

• KADOWAKI Setsu

  Department of Food Science and Technology, Kyoto University

• YAMAMOTO Kenji

  Department of Food Science and Technology, Kyoto University

• KUMAGAI Hidehiko

  Department of Food Science and Technology, Kyoto University

• TOCHIKURA Tatsurokuro

  Department of Food Science and Technology, Kyoto University

書誌事項

タイトル別名

• Purification and characterization of endo-.ALPHA.-N-acetylgalactosaminidase from Alcaligenes sp.

抄録

A gram negative bacterium isolated from soil was found to produce an endo-α-N-acetylgalactosaminidase in culture. The microorganism was identified as Alcaligenes sp. from various bacteriological characteristics. The enzyme was purified from the culture fluid by fractionation with ammonium sulfate, and column chromatographies on DEAE-Sephadex A-50 and hydroxylapatite. The molecular weight of the enzyme was estimated to be 160, 000 by gel filtration and SDS-polyacrylamide gel electrophoresis. The optimum pH was found to be 4.5 and the stable pH range was 4.5-6.5. The Km values were 3.7mM and 3.2mM with asialofetuin and asialo κ-casein glycopeptide as substrates, respectively. The enzyme released the disaccharide, Galβ1→3GalNAc, from glycoproteins possessing serine or threonine O-glycosidic linkages. The enzyme production was highly induced by porcine gastric mucin added to the medium.

収録刊行物

• Agricultural and Biological Chemistry

  Agricultural and Biological Chemistry 52 (7), 1715-1723, 1988

  公益社団法人 日本農芸化学会

キーワード

• General Agricultural and Biological Sciences
• General Biochemistry, Genetics and Molecular Biology

詳細情報

• CRID: 1390001206464237696
• NII論文ID: 110006323887
• NII書誌ID: AA00515312
• DOI: 10.1271/bbb1961.52.1715
• COI: 1:CAS:528:DyaL1cXls1KitLo%3D
• ISSN: 18811280
• ISSN: 00021369
• ISSN: http://id.crossref.org/issn/00021369
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可